Generator

Part:BBa_K2012026:Design

Designed by: Boyao Zhang   Group: iGEM16_HZAU-China   (2016-10-07)


PcpcG2-B0034-PleD-His(6)Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 278
    Illegal BamHI site found at 1436
    Illegal XhoI site found at 1630
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 694
    Illegal NgoMIV site found at 841
    Illegal AgeI site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 351


Design Notes

The expression of PleD can be activated under the control of green light. His(6)tag is added to PleD so that the recombinant protein can be purified through Ni-chelating affinity chromatography.


Source

PcpcG2 comes from the genome of Synechocystis PCC6803(Part BBa_K592003). PleD from Caulobacter crescentus, a response regulator with a diguanylate cyclase (DGC) domain.(Part:BBa_K2012002)

References